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The goal of this computer vision software is to take the guesswork out of grapple operations aboard the ISS by providing a robotic arm operator with real-time pose estimation of the grapple fixtures relative to the robotic arms end effectors. To solve this Perspective-n-Point challenge, the software uses computer vision algorithms to determine alignment solutions between the position of the camera eyepoint with the position of the end effector as the borescope camera sensors are typically located several centimeters from their respective end effector grasping mechanisms. The software includes a machine learning component that uses a trained regional Convolutional Neural Network (r-CNN) to provide the capability to analyze a live camera feed to determine ISS fixture targets a robotic arm operator can interact with on orbit. This feature is intended to increase the grappling operational range of ISSs main robotic arm from a previous maximum of 0.5 meters for certain target types, to greater than 1.5 meters, while significantly reducing computation times for grasping operations. Industrial automation and robotics applications that rely on computer vision solutions may find value in this softwares capabilities. A wide range of emerging terrestrial robotic applications, outside of controlled environments, may also find value in the dynamic object recognition and state determination capabilities of this technology as successfully demonstrated by NASA on-orbit. This computer vision software is at a technology readiness level (TRL) 6, (system/sub-system model or prototype demonstration in an operational environment.), and the software is now available to license. Please note that NASA does not manufacture products itself for commercial sale.