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In vitro three-dimensional (3D) human broncho-epithelial (HBE) tissue-like assemblies (3D HBE TLAs or TLAs) were engineered in modeled microgravity using rotating wall vessel technology (pictured above) to mimic the characteristics of in vivo tissue. The TLAs were bioengineered onto collagen-coated cyclodextran beads using primary human mesenchymal bronchial-tracheal cells (HBTC) as the foundation matrix and an adult human broncho-epithelial immortalized cell line (BEAS-2B) as the overlying component. The resulting TLAs share significant characteristics with in vivo human respiratory epithelium including polarization, tight junctions, desmosomes, and microvilli. The presence of tissue-like differentiation markers including villi, keratins, and specific lung epithelium markers, as well as the production of tissue mucin, further confirm these TLAs have differentiated into tissues functionally like in vivo tissues. TLAs mimic aspects of the human respiratory epithelium and provide a unique capability to study the interactions of respiratory viruses and their primary target tissue independent of the host's immune system. The innovation "Methods For Growing Tissue-Like 3D Assemblies Of Human Broncho-Epithelial Cells" is at Technology Readiness Level (TRL) 6 (which means system/subsystem prototype demonstration in a relevant environment) and the related patent is now available to license for development into a commercial product. Please note that NASA does not manufacture products itself for commercial sale.

The innovative technology from Ames acts as a microfluidics switch array, using combinations of pressure and flow conditions to achieve specific logic states that determine the sequences of sample movement between microfluidic wells. This advancement will enable automation of complex laboratory techniques not possible with earlier microfluidics technologies that are designed to follow predetermined flow paths and well targets. This novel method also enables autonomous operations including changing the flow paths and targeting well configurations in situ based on measured data decision parameters. This microfluidic system can be reconfigured for use in various experimental applications, requiring only an adjustment of the programmed pressure sequencing, reducing the need for custom design and development for each new application. For example, this technology could provide the ability to selectively constrain or move biological specimens in the experimental wells, allowing evolutionary studies of model organisms in response to various stressors, evaluation of different growth conditions on biological production of antibodies or other small molecule therapeutics, among other potential applications. Likewise, this platform can be used to foster time-dependent, step-wise, chemical reactions, which could be used for novel chemical processes or in situ resource utilization.

In the development of this technology for the ISS, engineers had to pay careful attention to electrical draw efficiency, ease-of-use, mass reduction, production cost, and safety, as conducting scientific research under spacecraft stressors is an important requirement. To create a controlled environment within the science enclosure, engineers designed a ventilation system incorporating an external fan/blower that pulls air across a HEPA filter and diffuses it in a manner that creates an even laminar flow within the enclosure before exiting through the exhaust filter. The glove seal forms an airtight and liquid impervious seal. This novel design also allows the user flexibility to choose their own task-specific glove material, facilitates easy tool-free assembly and quick glove changes, and may be transferable to other types of enclosures. Another key feature is that a through-port can be quickly fitted to an empty glove port. Due to the science enclosure system intended application aboard the ISS, its electrical draw does not exceed 24V, thereby making it feasible to power it from a battery for terrestrial field use or other applications where accessing power is a challenge. The combination of its performance, portability, BSL 2 capability, and inexpensive production costs could position the science enclosure system and accompanying innovations to be valuable in the fields of education, research, clean rooms, hospitals, and disaster relief efforts.