Surface Attached BioReactor (SABR) for Microbial Cell Cultivation

The miniature bioreactor system was developed to provide the capabilities for NASA to perform cell studies in space and then provide results back to investigators on Earth with minimal tools and cost. The miniature bioreactor system has the potential to also be used on Earth as a laboratory bench-top cell culturing system without the need for expensive equipment and reagents. The system can be operated under computer control to reduce the operator handling and to reduce result variations. The system includes a bioreactor, a fluid-handling subsystem, a chamber wherein the bioreactor is maintained in a controlled atmosphere and temperature, and control subsystems. The system can be used to culture both anchorage dependent and suspension cells (prokaryotic or eukaryotic cell types). Cells can be cultured for extended periods of time in this system, and samples of cells can be extracted and analyzed at specified intervals. The miniature bioreactor system for cell culturing has applications in pharmaceutical drug screening and cell culture studies.

PONDS was developed as a water/nutrient delivery system for the Vegetable Production System, called VEGGIE, on the International Space Station (ISS). PONDS uses an innovative wicking material to passively link a water/nutrient reservoir to a plant cylinder. The system enables higher germination rates and improved growth conditions compared to the VEGGIE water/nutrient delivery system currently used on the ISS. PONDs consists of two primary components: a water/nutrient reservoir (Figure 1), and a detachable plant cylinder containing growth substrate and wicking material (Figure 2). The reservoir includes a viewing window that allows the user to observe and record water-use data. The plant cylinder, which screws into the reservoir system, is made from commercial-off-the-shelf materials and fittings. Both the reservoir and plant cylinder include oxygen-permeable windows to enhance aeration to the root zone. Water is delivered from the reservoir to the substrate contained within the plant cylinder via the wicking material inserted into the growth substrate. The wicking material is intrinsically hydrophilic, providing improved capacity compared to the system previously used with VEGGIE. As a result, PONDS can continuously supply water to the root zone within the plant cylinder on demand.

Because resupply of commodities for long duration space missions would be prohibitively expensive and could take an extensive length of time to reach habitats in orbit around or on other planetary bodies, it is critical that astronauts have the ability to recycle and reuse local waste streams to provide resources such as clean water, fuel, and nutrients for growing plants. Scientists at Kennedy Space Center and the University of South Florida have developed a technology that addresses this critical mission need. The modular system design incorporates all wastewater streams and some food waste including urine water, hygiene water, humidity condensate, Sabatier water, fecal waste, laundry water, and organic food waste. These sources are fed simultaneously into the system, and a function-driven, sequential purification process occurs. The primary processes include carbon conversion, phase separation (solid/liquid/gas), disinfection, nutrient/salts management, and salts balancing to generate a clean water stream. The heart of the closed-loop bio-regenerative system is an anaerobic membrane bioreactor (AnMBR), which takes raw wastewater streams and utilizes an anaerobic microbial consortium to carry out the breakdown of the organic matter. An ultrafiltration membrane captures and destroys pathogenic bacteria and viruses. The AnMBR system generates a clean water stream containing fertilizer constituents which can be used to cultivate either microalgae (for food, pharma/nutraceuticals, fuel or bioplastics) in photobioreactors or crops in hydroponic systems. The system also generates methane and hydrogen gas which can be used for fuel (or conversion to bioplastics), and CO2 which can be used to support plant growth.

Bacterial contamination of water systems used in microgravity is a major issue for NASA because biofilms can clog or interfere with water system functions and bacterial ingestion can be harmful to astronaut health. To address this problem, NASA innovators developed a microwave based technology to purify contaminated water by eradicating and eliminating bacteria that grows in systems that generate potable water, in equipment utilizing cooling loops and heat exchangers, and removing bacterial contamination that is present on a variety of surfaces. This decontamination system is chemical free and requires minimal to no consumables. Initial testing identified a specific microwave frequency band and exposure times for killing bacteria (Burkholderia cepacia) and biofilm. Test results show that exposing static water to microwave energy for 90 seconds can effectively kill waterborne bacteria and biofilm within a water filtration system. Additional testing, using a circulating water test bed, demonstrated that microwave energy at the selected frequency can effectively eradicate waterborne bacteria within 30 seconds. This technology could be further developed into a portable, lightweight system for use in remote locations as well as commercial space applications. The microwave decontamination system could also be added to existing water systems to extend the life of the system.

Once genes for a desired material type, delivery mode, control method and affinity have been chosen, assembling the genetic components and creating the cell lines can be done with well-established synthetic biology techniques. A 3D microdeposition system is used to make a 3D array of these cells in a precise, microstructure pattern and shape. The engineered cells are suspended in a printable 'ink'. The 3D microdeposition system deposits minute droplets of the cells onto a substrates surface in a designed print pattern. Additional printer passes thicken the material. The cell array is fed nutrients and reagents to activate the engineered genes within the cells to create and deposit the desired molecules. These molecules form the designed new material. If desired, the cells may be removed by flushing. The end product is thus a 3D composite microstructure comprising the novel material. This innovation provides a fast, controlled production of natural, synthetic, and novel biomaterials with minimum resource overhead and reduced pre- and post-processing requirements.