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NASAs single grid, self-aligned focusing schlieren optical assembly is attached to a commercial-off-the-shelf camera. It directs light from the light source through a condenser lens and linear polarizer towards a polarizing beam-splitter where the linear, vertically-polarized component of light is reflected onto the optical axis of the instrument. The light passes through a Ronchi ruling grid, a polarizing prism, and a quarter-wave plate prior to projection from the assembly as right-circularly polarized light. The grid-patterned light (having passed through the Ronchi grid) is directed past the density object onto a retroreflective background that serves as the source grid. Upon reflection off the retroreflective background, the polarization state of light is mirrored. It passes the density object a second time and is then reimaged by the system. Upon encountering the polarizing prism the second time, the light is refracted resulting in a slight offset. This refracted light passes through the Ronchi ruling grid, now serving as the cutoff grid, for a second time before being imaged by the camera. Both small- and large-scale experimental set ups have been evaluated and shown to be capable of fields-of-view of 10 and 300 millimeters respectively. Observed depths of field were found to be comparable to existing systems. Light sources, polarizing prisms, retroreflective materials and lenses can be customized to suit a particular experiment. For example, with a high speed camera and laser light source, the system has collected flow images at a rate of 1MHz.

The handheld digital microscope features a 3D-printed chassis to house its hardware, firmware, and rechargeable Li-ion battery with built-in power management. It incorporates an internal stainless-steel cage system to enclose and provide mechanical rigidity for the optics and imaging sensor. To reduce the microscope’s size, yet retain high spatial resolution, engineers devised an optical light path that uniquely folds back on itself using high reflectivity mirrors, thus significantly reducing internal volume. Imaging control and acquisition is performed using a secure web-based graphical user interface accessible via any wireless enabled device. The microscope serves as its own wireless access point thus obviating the need for a pre-existing network. This web interface enables multiple simultaneous connections and facilitates data sharing with clinicians, scientists, or other personnel as needed. Acquired images can be stored locally on the microscope server or on a removable SD card. Data can be securely downloaded to other devices using a range of industry standard protocols. Although the handheld digital microscope was originally developed for in-flight medical diagnosis in microgravity applications, prototypes were thoroughly ground-tested in a variety of environments to verify the accurate resolve of microbial samples for identification and compo-sitional analysis for terrestrial field use. Owing to its portability, other applications demanding rapid results may include research, education, veterinarian, military, contagion disaster response, telemedicine, and point-of-care medicine.