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The technology provides miniaturized techniques for extracting trace amounts of organic molecules (lipids) from natural samples. It operates as an autonomous, miniaturized fluidic system, integrating lab techniques for lipid analysis while minimizing reagent volumes and concentrating organics for analysis, thereby increasing signal-to-noise ratios by orders of magnitude. The non-aqueous fluidic system described herein for astrobiological and life-detection missions (either in situ or returned sample) is configured to extract lipid organics from regolith using (1) a fluidic sample processor made of materials compatible with organic solvents and (2) a machine-learning system to select processing steps and parameters to maximize lipid yield. A critical gap is bridged by integrating technologies into a system that replicates analytical lab procedures autonomously on a spaceflight instrument scale with fidelity to original lab techniques. Automated fluidic devices combine controlled handling of liquids with sequential operations and parallelization of replicate processes. By designing such systems to closely interface with both sample-delivery and analytical measurement systems, laboratory analyses are automated. The technology adapts best practice laboratory methods for lipid analysis, overcoming analytical challenges like low organic abundance, interference of minerals/salts, and degradation of origin-diagnostic molecular structures. The extraction and concentration techniques from rock/soil samples can be applied to any biomarkers by changing the solvent, temperature, and agitation.

NASA's new calibration system is a proprietary method to quickly design and make predictable and repeatable gap-and-overlap defects when employing AFP. The system creates defects within the course of layup with known sizes, geometries, and locations. Using this defect-creation technique, one can now accurately quantify the ability to detect defects on inspection systems, perform accurate risk assessments, and calibrate in-situ inspection equipment to specific materials. The equipment that makes the defects can be efficiently and inexpensively 3D printed. This technique is currently being used to successfully calibrate NASA's in situ inspection system for their AFP equipment. AFP is experiencing increasing adoption in aerospace, automotive, and other industries that leverage large-scale advanced composite components. NASA's new AFP calibration system could be very useful to companies that develop and manufacture AFP machines or AFP machine inspection equipment to improve the quality of their products in a provable manner. Furthermore, users of AFP machines may find value in the tool for creating their own calibration standards.

The jointly developed interlocking paver design consists of a molded solid material with tapered interlocking features that interface with features of an opposite gender in three orthogonal directions. This establishes a toleranced connection between the pavers that locks down six degrees of freedom. More specifically, the system consists of two types of pavers: polygon and spacer pavers. Both are symmetrical about the longitudinal and transverse axes and are designed to interlock securely with one another in a checkerboard pattern. The polygon paver features an octagonal top level and a rectangular bottom level with protrusions and recessed notches. The spacer paver has an elongated center portion with isosceles trapezoid extensions on the top level and a rectangular bottom level with protrusions and notches. The interlocking design locks down six degrees of freedom, providing enhanced stability and preventing the flow of exhaust gases between the seams to mitigate erosion of the underlying regolith. The pavers could be constructed leveraging in-situ resource utilization (ISRU). Lunar regolith has been identified as a potential construction material. Additionally, the pavers could be installed via robotic assembly, reducing the need for human labor in harsh environments.

The invention consists of a 3D print head apparatus that heats and extrudes a regolith-polymer (or other) mixture as part of an additive manufacturing process. The technology includes a securing mechanism, hopper, nozzle, barrel, and heating system. The securing mechanism attaches to a wrist joint of a robotic arm. The hopper, connected to the securing mechanism, has a cavity and a lower aperture. The barrel is an elongated, hollow member with its first end connected to the hopper's lower aperture and its second end connected to the nozzle's upper aperture. The heating system is positioned along the barrel and comprises a heater, thermocouple, insulator, and heating controller. The heating controller activates the heater based on input signals received from the thermocouple. The print head apparatus also includes a feed screw, drive shaft, and motor. The feed screw is positioned within the elongated hollow member of the barrel, and the drive shaft transmits torque to the feed screw. The motor provides torque to the drive shaft. An agitator is secured to the drive shaft, facilitating the consistent movement and mixing of the regolith-polymer mixture in the hopper. The nozzle includes a tube with an open end and an occluded end, allowing the mixture to be extruded through the lower aperture. The jointly developed 3D print head technology enables efficient, large-scale additive construction using in-situ resources, such as regolith or other materials. The innovation reduces the need for transporting materials from Earth and allows for sustainable habitat development on the Moon or Mars. Given its adaptability to different crushed rock-polymer materials, the invention may also serve as an alternative to conventional Portland concrete construction on Earth.

Modular Artificial-Gravity Orbital Refinery Spacecraft is a solution for refining in-situ materials collected in space, such as from asteroids and Mars moons, as well as recycling spacecraft debris, while orbiting in micro-gravity conditions. The spacecraft is coupled with refining modules for refining and recycling different types of materials. It generates artificial gravity for operation in low-gravity environments. The spacecraft is comprised of rotating rings, each generating artificial gravity and angular momentum. When the rotating rings are combined on the spacecraft platform, however, they have a net near-zero angular momentum such that the spacecraft can change its attitude with minimal propellant or rotate at the rate of the object the spacecraft platform is attached to. The spacecraft platform can self-balance to accommodate different sized modules and modules with moving loads. The refined and recycled materials can be used to create products in-situ as well as products too large to launch from Earth, such as construction of orbiting space habitats, large spacecraft, solar-power stations, and observatories.

The technology is a solid state, Carbon Dioxide (CO2) sensor configured for sensitive detection of CO2 having a concentration within the range of about 100 Parts per Million (ppm) and 10,000 ppm in both dry conditions and high humidity conditions (e.g., > 80% relative humidity). The solid state CO2 sensor achieves detection of high concentrations of CO2 without saturation and in both dynamic flow mode and static diffusion mode conditions. The composite sensing material comprises Oxidized Multi-Walled Carbon Nanotubes (O-MWCNT) and a metal oxide, for example O-MWCNT and iron oxide (Fe2O3) nanoparticles. The composite sensing material has an inherent resistance and corresponding conductivity that is chemically modulated as the level of CO2 increases. The CO2 gas molecules absorbed into the carbon nanotube composites cause charge-transfer and changes in the conductive pathway such that the conductivity of the composite sensing material is changed. This change in conductivity provides a sensor response for the CO2 detection. The solid state CO2 sensor is well suited for automated manufacturing using robotics and software controlled operations. The solid state CO2 sensor does not utilize consumable components or materials and does not require calibration as often as conventional CO2 sensors. Since the technology can be easily integrated into existing programmable electronic systems or hardware systems, the calibration of the CO2 sensor can be automated.

To stain a specimen slide, one or more liquid reagents are injected via the dispenser into the slide staining device via a syringe port. The volume of a given reagent is determined by adjustable settings on the dispenser, so that when connected to the staining device, initiates a thin film over the slide. The dispensing device uses only a fraction of the reagents typically used in non-sealed environments. Medical grade polyvinyl alcohol sponges have been incorporated into the dispenser to provide additional fluid containment and retention during the staining procedure. Furthermore, the dispenser can recall excess reagent, minimizing reagent use until refill. The slide staining device is composed of an upper and lower section held together and aligned by use of Nd magnets. With the device open, a specimen slide is positioned upon a silicone gasket that sits within a recess in the lower section. When the device is closed, the silicone gasket in the upper section applies a seal to the slide forming a cavity that allows the slide to be exposed to reagents injected from the connected dispenser creating a stain through the use of capillary forces. Although originally designed for use in microgravity, the slide staining system also works in gravity environments. Numerous applications may exist for this technology, particularly in hematology and cellular biology. Other applications could be considered for academic research, veterinary field use, military, disaster stricken and remote environments or where fine control of fluid delivery, removal, and management is desired. The slide staining system is at technology readiness level (TRL) 8 (actual system completed and "flight qualified" through test and demonstration), and are now available to license. Please note that NASA does not manufacture products itself for commercial sale.